Composite

Part:BBa_K3748049:Design

Designed by: Sofía Jiménez Ochoa   Group: iGEM21_Groningen   (2021-10-01)


pREV1-αMF-BS-tTDH1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1590
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1590
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2841
    Illegal EcoRI site found at 1590
    Illegal BglII site found at 705
    Illegal BglII site found at 2062
    Illegal BglII site found at 2232
    Illegal BamHI site found at 2082
    Illegal XhoI site found at 954
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1590
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1590
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Golden Gate


Source

MoClo Kit and Uniprot

References

Lee, M. E., Deloache, W. C., Cervantes, B., & Dueber, J. E. (2015). A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol., 4, 975–986. https://doi.org/10.1021/sb500366v

https://www.uniprot.org/uniprot/P00691 - Alpha amylase B. subtilis